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Ozone Therapy Retinitis Pigmentosa

Ozone Therapy Retinitis Pigmentosa

Ozone Therapy Retinitis Pigmentosa

Ozone Therapy in Patients with Retinitis Pigmentosa

 Mirtha Copello1, Frank Eguía2, Silvia Menéndez*3, Niusdalys Menéndez1
 1 Retinitis Pigmentosa National Reference Center. “Dr. Salvador Allende” Hospital. Calzada
del Cerro Ave. and Domínguez, Cerro, Havana, Cuba
2 ” Ramón Pando Ferrer” Ophthalmologic Hospital. 76 St. and 31 Ave. Marianao, Havana, Cuba
3 Ozone Research Center. POBox 6880, Havana. Cuba

 Abstract

Retinitis pigmentosa (RP) is characterized by progressive night blindness. The aim of this study is to determine the efficacy of ozone therapy retinitis pigmentosa. A controlled, randomized, double blind clinical trial involving 68 patients was performed. Patients were divided into 2 groups: ozone, patients were treated with ozone by rectal administration  (dose=10 mg), during  15 sessions; control, as ozone group, but using oxygen. The main outcome variable was the visual field area (VFA). Results demonstrated a significant improvement (SI) in 88.2 % of patients treated with ozone in comparison with 23.5 % achieved in the control group. In the ozone group, VFA tend to stabilize beyond a mean time of 6.83 months with a loose in SI afterward. A temporal positive effect of ozone therapy, over the natural course of RP, was found. It could be useful to apply ozone therapy in the first stages of the disease and at six-month intervals in order to enhance visual capabilities in RP patients.

Introduction

Retinitis Pigmentosa (RP) is the name usually applied to a diverse group of hereditary retinal degeneration, with an overall prevalence of about 1/4000 (1) and about 1 500 000 affected people  worldwide.  Inheritance  could  be  autosomal  dominant,  recessive,  sex  linked, mitochondrial, digenic or frequently isolated cases are presented. Patients complain of night blindness and  progressive  reduction  of  peripheral  vision (visual  field  loss)  with  a  ring scotoma, with late involvement of central vision and abnormal electroretinogram (2,3). About 50 % of patients with RP are legally blind at middle age, most of them as a result of visual field reduction (4).

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Ozone Therapy Methyl Linoleate

Ozone Therapy Methyl Linoleate

Ozone Therapy Methyl Linoleate

Effect  of  α-Tocopherol  During  in  Vitro  Ozonation  of Methyl Linoleate. Its Implication in Ozone Therapy.

 Maritza F. Díaz Gómez1; José A. Gavín Sazatornil2; Frank Hernández Rosales1 and Wilfredo Díaz1 Rubí.
Ozone Research Center. National Center for Scientific Research. Havana, Cuba. P.O. Box 6412,  Havana,  Cuba.
2University  of  the  Laguna 38207, Tenerife, Spain.

 Abstract

Systemic ozone therapy is widely used as an oxidant therapy to restore many troubles and diseases. It is known that ozone therapy works through a transient oxidative stress that relays the beneficial effects to long distance from the application site. The convenience of supporting patients with antioxidants during systemic ozone therapy applications is now under discussion. We studied the reaction of linoleate (one of the main constituents of cellular membranes and plasma phospholipids) with ozone in presence or absence of α-tocopherol, in order to explore whether the combination of ozone and antioxidant has some effect on those ozonation products that produce the transient oxidative stress.

The reaction was followed by Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR) using 35 and  69 mg/L as ozone concentrations, and  50,  90, and  130 micromolar α-tocopherol concentrations with 0.0048 M of methyl linoleate. Ozonide (δ = 5.2 ppm) and aldehydes (δ = 9.63 ppm and δ = 9.74 ppm) intensities from 1H-NMR signals markedly decreased with α-tocopherol addition. When α-tocopherol is absent, the intensities from olefinic proton signals diminished with ozone concentration increment; however, with α-tocopherol in the mixture a smaller decrement was achieved. No detectable signals were found with the ozonation of α-tocopherol without methyl linoleate in the reaction mixture. These results suggest that α-tocopherol reacts with ozonation products from the reaction of ozone with methyl linoleate. This fact point out that antioxidant supplementation during systemic ozone  therapy (major  and  minor  autohemotherapy,  rectal  insufflation,  etc)  can  be detrimental in achieving the needed transient oxidative stress.

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Medical Ozone Threatened Abortion

Medical Ozone Threatened Abortion

Medical Ozone Threatened Abortion

The Experimental Grounds for Safe and Effective Dosage
     of Medical Ozone used in the Treatment of Abortion

Guennadi O. Gretchkanev1, Claudia N. Kontorchikova2, Tatyana S. Katchalina3, El-Hassoun Husein4
1,3 Department of Obstetrics and Gynecology, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
2 Central Research Laboratory, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
4 Russian Association of Ozone Therapy, Jordan

 Abstract

Blood samples of 15 patients with threatened abortion, 15 healthy non-pregnant women  and  15  pregnant  women  without  pathology  were  investigated.  The following indices were determined: the antioxidative plasma activity (by method of  bio chemiluminescence),  the  level  of  diene  conjugates (by  method  of spectrophotometry) and the Schiff’s bases (after D.I.Flether).

The influence of ozone on the above indices depended on the used concentration of ozone. The ozone concentration of 400 mcg/L induced a significant increase (by 2,6 times) in the antioxidative plasma activity. Meanwhile, the level of lipid peroxidation  molecular  products  had  a  tendency  to  decrease.  Higher  ozone concentrations (800 to 1200 mcg/L) led to reverse processes.

Introduction

For over 100 years ozone has been successfully used in various fields of medicine, but till 1993 in literature there were no references to ozone applications in the treatment of obstetric complications, that was why for safe clinical applications of medical ozone there was a need for experimental grounds of the present method of treatment. The aim of our investigation was to establish in vitro a range of ozone concentrations being safe for the homeostasis of patients with threatened abortion for the purpose of its further using in clinical conditions.

Materials and Methods

As a criterium in vitro for safety and efficiency of the given ozone concentrations it was considered intensification of the antioxidative defense system (AODS) and a decrease in the molecular products of lipid peroxidation (LP). The choice was based on the objectivity and informativity of the above indices as well as their importance for prognosis of obstetric pathologies including threatened abortion (2,3,5,6).

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Ozone Therapy Female Genital Organs

Ozone Therapy Female Genital Organs

Ozone Therapy Female Genital Organs

The New Method of Treatment of Inflammatory Diseases of  Lower Female Genital Organs

Guennadi O. Gretchkanev1, Tatyana S. Katchalina2, Olga V. Katchalina3, El-Hassoun Husein4
1,2,3Department of Obstetrics and Gynecology, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
4 Russian Association of Ozone Therapy, Jordan

Abstract

For treatment of  40 patients with non-specific colpitis we used a method of streaming ozone therapy with assistance of the ozone generator MEDOZONS-BM and special attachment on the basis of vaginal speculum. The irrigation with an ozone-oxygen mixture with ozone concentration of 2000 mcg/L was carried out within  5 minutes after preliminary moistening of mucosa with sterile distilled water.

In the course of investigations it was established that along with a positive clinical effect ozone therapy caused an improvement in vaginal local immunity indices – a credible increase in lysozyme in 70% of patients, a decrease in IgG level in 60% of patients, stabilization of cervical mucus myeloperoxidase in 80% of patients.

Introduction

The  problem  of  infectious  diseases  and  dysbiosis  nowadays  acquires  particularly  great importance  that  is  connected  with  growing  ecological  problems  of  urbanized  society, exposure to various adverse factors having influence on the immunity status in general as well as micro biocenosis of human body. It is established that the pathological processes of vaginal micro biocenosis are caused by such stress factors as treatment with antibiotics (at local and systemic  level),  hormones,  cytostatic  preparations,  X-ray  therapy,  particularly  on  the background of endocrinopathies (most of all, diabetes), anemia, in case of birth defects of genital organs, the use of contraceptives and so on (1). It is convincingly demonstrated that the disturbances of a quantitative correlation between the association of vaginal micro biocenosis on the background of local immunity changes lead to clinical appearances of vaginal infection process, the most frequent of them are non-specific vaginitis and bacterial vaginosis (1,3). Bacterial vaginosis is detected in 24% of practically healthy women and in 61% of patients with recidivating colpitis of nonspecific etiology (2), frequency of nonspecific vaginitis, in reference to different authors, reaches 40 to 50%. The extensive use of new antibiotics does not solve the problem, but in some cases aggravates it by inducing the disturbances of the local and  general  immunity,  by  suppressing  saprophytic  flora,  by  causing  the  formation  of antibiotic-resistant forms of microorganisms, by creating favourable conditions for infection that increases frequency of recidivating colpitis. In this connection, our attention is drawn to the methods of treatment which can be characterized as highly effective against the most frequent causative agents of infection, able to reduce antibiotic and any other drug therapies and able to restore the normal vaginal biocenosis.

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