The Mechanism of the Anti-Inflammatory Effect of Ozonated Physiological Saline
Guennadi O. Gretchkanev1, Natalia M. Shachova2, A.F. Nevmyatullin3, Tatyana S.
Katchalina4, N.N. Saifieva5, El-Hassoun Husein6
1,2,3,4,5 Department of Obstetrics and Gynecology, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
6 Russian Association of Ozonetherapy, Jordan
The ozonated 0,9% sodium chloride solution barbotaged in a standard bottle by ozone-oxygen mixture with ozone concentration of 1200 mcg/L for 15-20 minutes at flow rate 1 L/min produces a positive effect on the clinical course of acute PID and on some indices of the homeostasis in the given group of patients when introduced intravenously.
The fungicidal and bactericidal effects of ozonated saline have not been established during the experiments in vitro on pure cultures of Staphylococcus aureus CCM 885 gram-positive bacteria, Escherichia coli M-17 gram-negative bacilli and Candida albicans yeast-like fungi.
The study of the spontaneous (SCL) and induced chemiluminescence (ICL)parameters of neutrophils in patients with acute PID testifies to the ability of ozonides to stimulate the function of polymorphonuclear leukocytes.
Acute uterine appendages inflammation or pelvic inflammatory disease (PID – according to International classification) is the most frequent pathology among the women of reproductive
age, it is detected in 60-65% of gynecological patients, and 20-30% of them need hospital treatment.
The complex conservative treatment of acute PID includes therapeutic methods which service not only to eliminate the causative agent of disease, but also to correct the metabolic and immunological disturbances.
Materials and Methods
The aim of the experiment in vitro was to investigate the bactericidal properties of ozonated physiological saline solution produced by barbotage method by introducing an ozone-oxygen
mixture with ozone concentration of 1200 mcg/L into the saline. The investigation was conducted on pure cultures of S. aureus, E. coli and C. albicans. 18 hour broth cultures were
sowed out by lawn method on growth agar in Petri dishes, after that 20,0 ml of ozonated…