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Medical Ozone Dentistry

Medical ozone dentistry

Medical Ozone Dentistry

by Dr. Julian Holmes

The World Ozone Conference was held in Imperial College in September 2001 and the topic was Medical Applications of Ozone. Dentistry is far behind other fields of medicine in introducing ozone for therapeutic purposes.

Studies have shown that just 10 seconds of exposure to ozone (O3) can eliminate the number of colony-forming bacteria. The niche where bacteria has become established has taken a long time to develop and has become the exclusive domain of acid-loving bacteria. Ozone destroys this niche, destroying the bacteria, and also breaking up their metabolites so the whole lesion is effectively sterilized.

Ozone therapy also destroys the protein coat over the lesion, which effectively ‘protects’ the niche. Saliva contains all the bio-available minerals the tooth needs to remineralise. It cannot, due to the acid conditions, and the protein coat. By removing this, the natural minerals can enter the lesion, and effectively seal it. So, provided we set up the right conditions, you could argue that there is no need to place a filling, and my study showed this.

For years, we have been told to isolate cavities prior to placing a filling. Now ozone technologies may turn this upside down, by saying that prior to filling, you ozone first, let the patient lick the cavity area, and then isolate, re-ozone, then place your filling. Why do we need the contamination? For its mineral component, of course. What better dental sealant can you offer the patient? Whilst this research is not yet complete or published, this may be the way future filling replacement is going to be directed.

Lastly, look at water line cleanliness. An average dental unit has about 20 million bacteria, and a lot of them are a real threat to immuno-compromised patients. One flushing with ozonated water reduces the bacteria count to about 8,000 / ml, and after 12 hours, NOT A SINGLE bug can be cultivated. Research in Belfast has shown that the biofilm is stripped out of the entire system. So now we can say it is possible to sterilise a dental unit, not just clean it.

Ozone technologies are here to stay. Dentistry is catching up with our medical colleagues who have used this technology for about 100 years.We can stabilise the base first through a simple procedure, to minimise the volume of tooth tissue that has to be removed. We could put in place the right environment so that the decay stops, and is completely reversed.With a single 40 or 60 second application of ozone, we get complete and permanent pain relief. And there are many studies to show once tissue has remineralised, it never decays again.

I have already used ozone to treat 1275 lesions. To date, not a single one treated with ozone has got worse, and over 99% of those treated have reversed.In the control group, about 6 have shown any signs of reversal, and all the rest have got worse.

If you want to read the medical ozone dentistry research, you can download it from as well as recent articles.

There are many studies starting to look at this technology and its uses in other areas. Professor Lynch and his group have data for two applications. As the other applications are research and published, so they will be published in the dental journals.


1. Baysan A., Whiley RA., Lynch E. Antimicrobial effect of a novel ozone-generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res. 2000;34:498-501.

2. Baysan A., Lynch E. and Grootveld M. The use of ozone for the management of primary root carious lesions. Quintessence Publishing Group 2001;49-67.

3. Holmes, J, Lynch E., Clinical Reversal of Primary Occlusal Fissure Carious Lesions (POFCLs) using Ozone in General Dental Practice

4. Inaba D, Duscher H, Jongebloed W, Odelius H, Takagi 0, Arends J:The effects of a sodium hypochlorite treatment on demineralised root dentin. Eur J oral Sci 1995; 103: 368-374.

5. Inaba D, Ruben J, Takagi 0, Arends J:Effects of sodium hypochlorite treatment on remineralization of human root dentine in vitro. Caries Res 1996; 30: 214-218.

6. Effect of Ozone on Biofilms in Dental Unit Waterlines AL SHORMAN H1*, COULTER WA2, ABU-NBA’A L1, LYNCH E1.Restorative Dentistry, 2 Microbiology, School of Dentistry, Queen’s University Belfast, NI

7. Clinical reversal of root caries using ozone AYLIN BAYSAN, EDWARD LYNCH Journal of Dental Research

8. Oxidative Consumption of Biomolecules by Therapeutic Levels of Ozone MD TURNER, M GROOTVELD, CJL SILWOOD, E LYNCH Journal of Dental Research

9. Oxidising actions of an Anti-Bacterial Ozone-Generating Device towards Root Caries Biomolecules E LYNCH, CJL SILWOOD, C SMITH, M GROOTVELD Journal of Dental Research

10. Patients’ Attitudes to Managing Caries with Ozone H DOMINGO, C SMITH, R FREEMAN, E LYNCH Journal of Dental Research

11. The Effect of Ozone Application on Fissure Caries QLF Readings LAYLA ABU-NABA’A, HISHAM AL SHORMAN, EDWARD LYNCH Journal of Dental Research

12. Oxidative Modification of Salivary Biomolecules with Therapeutic Levels of Ozone AWD CLAXSON, C SMITH, MD TURNER, CJL SILWOOD, EDWARD LYNCH, MG ROOT VELD Journal of Dental Research

13. Ozone, An Effective Treatment For Dental Unit Water Lines HISHAM M AL SHORMAN, LAYLA ABU-NABA’A, WILSON A COULTER, EDWARD LYNCH Journal of Dental Research

14. Rapid Detection of Microbial-Derived Components in Dental Unit Water Lines by NMR Analysis C SMITH, H AL SHORMAN, M GROOTVELD, E LYNCH, BP MILLS, CJL SILWOOD Journal of Dental Research

15. Oxidative Consumption of Salivary Biomolecules by a Mouthrinse M. GROOTVELD*, E. LYNCH, A. CLAXSON, B. MILLS, and C.J. SILWOOD Journal of Dental Research

16. Oxidation of Root Caries Biomolecules by an Antibacterial Ozone-generating Device E. LYNCH*, A. BAYSAN, C.J. SILWOOD, B. MILLS, and M. GROOTVELD Journal of Dental Research

17. Oxidation of Human Plaque Biomolecules by an Antibacterial Ozone-generating Device B. MILLS*, E. LYNCH, A. BAYSAN, C.J. SILWOOD, and M. G ROOT VELD Journal of Dental Research

18. Management of Primary Root Caries Lesions Using Ozone {in vivo}A. BAYSAN* and E. LYNCH Journal of Dental Research

19. Therapeutic Salivary Oxidizing Activity of a Novel Antibacterial Ozone-generating Device C.J. SILWOOD*, E. LYNCH, A. BAYSAN, B. MILLS, and M. G ROOT VELD Journal of Dental Research

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