Medical Ozone Red Blood Cells
The Influence of Ozone on 2,3-Diphosphoglycerate Synthesis in Red Blood Cell Concentrates
Allhard Hoffmann1 and Renate Viebahn2
Research Centre of Medical Technology and Biotechnology Bad Langensalza,
Dept. fzmb-diagnostic Erfurt, Blumenstr. 70, D-99092 Erfurt
2Dr. J. Hänsler GmbH, Nordring 8, D-76473 Iffezheim
The longer blood preserves are stored, the more haemoglobin losses, after transfusion, its ability to transfer oxygen from the lungs to the tissue. This is due to an increase in a physiologically abnormal oxygen affinity of haemoglobin from a rapid loss during storage of 2,3-diphosphoglycerate (DPG), causing a reduction in blood preserve quality. A rise in the oxygen affinity of Hb in patients with functionally restricted compensatory mechanisms is of pathophysiological importance. Developing methods for restoring the function of stored RBCs is important for tissue oxygen supply.
This investigation aimed at shifting the oxygen affinity of the RBCs back within a therapeutically favorable range by suitable treatment. Refunctionalisation of RBCs in preserves is described in a patent by Viebahn (1992) describing a treatment of stored preserves with an O3/O2 mixture producing an increase in erythrocytic DPG content. To examine this effect, cooled concentrates stored between 15 and 35 days received defined and controllable treatment, samples being transferred to a medium simulating post transfusion conditions. To assess the function and metabolic status of the RBCs, parameters were recorded to document this regeneration of their cellular metabolism. It was possible to show a significantly accelerated synthesis of DPG (10 – 30 %) in ozone-pretreated RBCs compared with untreated preserves.More