All posts in Ozone And Red Blood Cell Concentrates

Medical Ozone Red Blood Cells

Medical ozone red blood cells

Medical Ozone Red Blood Cells

The Influence of Ozone on 2,3-Diphosphoglycerate Synthesis in Red Blood Cell Concentrates

Allhard Hoffmann1 and Renate Viebahn2

Research Centre of Medical Technology and Biotechnology Bad Langensalza,

Dept. fzmb-diagnostic Erfurt, Blumenstr. 70, D-99092 Erfurt

2Dr. J. Hänsler GmbH, Nordring 8, D-76473 Iffezheim


The longer blood preserves are stored, the more haemoglobin losses, after transfusion, its ability to transfer oxygen from the lungs to the tissue. This is due  to  an  increase  in  a  physiologically  abnormal  oxygen  affinity  of haemoglobin from a rapid loss during storage of  2,3-diphosphoglycerate (DPG), causing a reduction in blood preserve quality. A rise in the oxygen affinity  of  Hb  in  patients  with  functionally  restricted  compensatory mechanisms is of pathophysiological importance. Developing methods for restoring the function of stored RBCs is important for tissue oxygen supply.

This investigation aimed at shifting the oxygen affinity of the RBCs back within   a   therapeutically   favorable   range   by   suitable   treatment. Refunctionalisation of RBCs in preserves is described in a patent by Viebahn (1992) describing a treatment of stored preserves with an O3/O2 mixture producing an increase in erythrocytic DPG content. To examine this effect, cooled concentrates stored between  15 and  35 days received defined and controllable treatment, samples being transferred to a medium simulating post transfusion conditions. To assess the function and metabolic status of the RBCs,  parameters  were  recorded  to  document  this  regeneration  of  their cellular  metabolism.  It  was  possible  to  show  a  significantly  accelerated synthesis of DPG  (10  –  30  %) in ozone-pretreated RBCs compared with untreated preserves.

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