All posts in Ozone And Sepsis

Ozone Therapy Sepsis

Ozone therapy sepsis

Ozone Therapy Sepsis

Ozone Prophylactic Effect and Antibiotics as a Modulator of Inflammatory Septic process in Rats

Zullyt B. Zamora 1, S. Menéndez 1, M. Bette 2, R. Mutters 3, S.Hoffmann 4 and S. Schulz 4 Ozone Research Center, Havana, Cuba.  2 Institute of Anatomy Cellular and Molecular Biology, University of Marburg, Germany. 3 Institute of Microbiology, University of Marburg, Germany. 4 Institute of Animals Laboratory, University of Marburg, Germany


192 Wistar male rats (180- 200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model.  The proinflammatory  cytokines  (IL-1β, TNFα, IL-6 and IL-2) expression were also determined in different haematopoietic organs. The animals were taken at random and divided into 16  groups, 12  animals  each:  Group  A,  positive  control (sepsis  without treatment); groups B and C treated with ozone (200 ug / 250 g b. w) and Cefotaxime  (25mg/kg), (0h, +1h)  and (+1  h)  respectively;  groups  D  and  E,  ozone  and Cefodizime (25mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9%. The other groups (J-P) of animals were treated only with antibiotics.

The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1β mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.

Read full document ozone therapy sepsis here

Share Button