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Ozone Inactivation Kinetics

Ozone inactivation kinetics

Ozone Inactivation Kinetics

Ozone Inactivation Kinetics of Multiple Antibiotic Resistant Strains of Bacteria in Water.

 S. Gutiérrez, I. Lezcano, Ch. Baluja and E. Sánchez  Centro de Investigaciones del Ozono
Calle 230 # 1313 y Avenida 15, Sioney, Playa, Apartado 6412 Ciudad Habana, Cuba.

Received for review:
Accepted for publication:


To determine the ozone inactivation kinetic of different Gram positive and Gram negative multiple antibiotics resistant bacterial strains from clinical isolation (CI), that resists too high concentration of chlorine, and compare them with reference strains was our  objective.  Microorganism’s  concentration  was  from 105  to 106  ufc/mL,  and chlorine and ozone concentrations were between 0.5 – 4 mg/L, and 0.36 – 2 mg/L respectively. Gram-positive multiple antibiotic resistant strains are the most resistant bacteria with chlorine. With ozone the multiple antibiotics resistant strains were more resistant that reference strains. There were not statistical differences on ozone resistant inside of strains of same species, neither between Gram positive and Gram negatives multiple antibiotic resistant strains, and for Escherichia coli the resistance not dipped of plasmid witnesses.


Of all environmental agents to which we are exposed, water is one of the most important being essential to life, and it is too the more commune infection way (De Marini et al., 1995). The oxidant property of ozone is used in water treatment as a strong germicidal agent (Lee et al., 1991; Laddie and Bland, 1990; Finch and Fatrbaim, 1991).

One of the most important problems in antibiotic therapy is the capability of bacteria to develop multiple antibiotic resistance. A problem of primary concern to public health to the presence of antibiotic resistant bacteria in water, is that they are eliminated by man and animal which eventually pollute water resources, swimming pools, etc. (El- Zanfaly, 1997). Chlorine treatment is the most commonly used method for controlling microorganisms, however chlorine and ultraviolet radiation have been reported to select for antibiotic resistant indicator microorganisms (Finch and Smith, 1987).

The objective of this work is to determine the ozone inactivation kinetics in water of different  Gram  positive  and  Gram  negative  multiple  antibiotic  resistant  bacterial strains from clinical isolation, that resist high concentration of chlorine and compare them with reference strains.

Read full document ozone inactivation kinetics here.

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