All posts in Ozone On Plasmodium Falciparum Infected Red Blood Cells

Ozone Therapy Infected Blood

Ozone Therapy Infected Blood

Ozone Therapy Infected Blood

The Effect of Ozone on Plasmodium Falciparum-Infected  Red Blood Cells

 Viebahn-Hänsler1, B. Lell2, P.G. Kremsner2
 1Dr.J. Haensler GmbH, Nordring 8, D-76473 Iffezheim, Germany
2Department of Parasitology, Institut for Tropical Medicine, University of Tuebingen,
Wilhelmstrasse 27, D-72074 Tuebingen, Germany


In  the  case    of  plasmodium-infected  red  blood  cells  we  have  been  able  to demonstrate for the first time that ozone has an effect on intracellular parasites without direct ozone/parasite contact, and without visible haemolysis. According to the initial parasitaemia, we found an inhibitive effect of ozone by factors between 3 and 7. The active mechanism involved can be explained via the reaction of ozone with the unsaturated fatty acids of RBC membrane and a consequent penetration of hydroxy hydroperoxides into the cells. The parasites are subjected to an increased oxidative stress, and their reproductive cycle is disrupted. Ozonation was carried out at a concentration of 80 µg/ml in a RBC suspension. Optimal growth inhibition was obtained by applying ozone twice, i.e. immediately before and after infection.

Introduction and Discussion of Problems

The highly efficient microbicide effect of ozone has been in worldwide use for over 100 years in wastewater treatment and drinking water disinfection, and the literature on this subject is correspondingly  extensive.  The  mechanisms  of  its  bactericidal  and  virostatic  or  virus-inactivating effect in vitro are well known, whereby the principal interest is directed at human
pathogenic microorganisms ( Masschelein 1996 and Brockmann and Botzenhardt 1999). All studies named, however, relate to surface waters and infected aqueous solutions which have for the most part had ozone/oxygen gas mixtures passed through them for a specific time until  obtaining the best  result.  In  this  way,  it  has  been  possible  to  determine  relevant concentration-time  concepts  in  the  case  of  most  microorganisms.  In  cases  where  these organisms are protected from ozone attack by external protein membranes or impurities, the combined application of ultrasound and ozone in addition to H2O2 with additional UV irradiation is effective.

We are faced with completely different problems in the case of intracellular microorganisms, which – protected by the outside cell membrane – are able to escape the direct attack of ozone, Plasmodium-infected red blood cells here being a typical example. Where the aim is to affect the reproductive cycle of intracellular microorganisms by using ozone without destroying the cellular structure of the host cell, careful ozone dose-finding and a standardized cell model is necessary. As the highly reactive O3 molecule is converted completely into peroxidic products in the red blood cell membrane, i.e. it is not able to penetrate  into  the  intracellular  space,  direct  contact  between  the  ozone  and  the  target microorganism is impossible.

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