Ozonated Unsaturated Triglycerides
Ozonized Unsaturated Triglycerides as Precursors of Urinary Dicarboxylic Acids
Daniel Jardines, Oscar Ledea and Zullyt Zamora, Ozone Research Center, National Center for Scientific Research, Ave 230 y 15, Playa, P.O. Box 6414, Havana City, Cuba.
Oral administration of ozonized sunflower oil to Wistar rats has produced changes in the urinary content of dicarboxylic acids. Heptanedioic acid (Pimelic acid) and nonanedioic acid (Azelaic acid) were the major increased dicarboxylic acids founded. The aim of this work is the study of the urinary dicarboxylic acid profiles of Wistar rats, orally treated with ozonized standard triglycerides. The dicarboxylic acids were extracted and derivatized before the analysis by Gas Chromatography – Mass Spectrometry. The urinary dicarboxylic acid profiles of the rats that received ozonized triolein, only showed the increasing of heptanedioic and nonanedioic acids. However, when ozonized trilinolein was applied, the profile is similar to that obtained, when ozonized sunflower oil was administered. A biochemical mechanism to explain the formation of dicarboxylic acids from ozonated unsaturated triglycerides was proposed.
Ozonized Sunflower Oil (OLEOZON®) is a registered drug that has shown antimicrobial effects against virus, bacteria and fungi (Cajigas et al., 1990; Lezcano et al., 1996; Lezcano et al., 1998). On the other hand, toxicological studies of OLEOZON® have demonstrated that this product is not mutagenic or genotoxic and has not secondary reactions in human patients (Llerena et al., 1995; Martinez et al., 1995; Remigio et al., 1998). Since 1994 it has being studied the use of ozonated sunflower oil in the treatment of giardiasis in animal models and in humans (Menéndez et al., 1995; Hernández, 1999).
Oral administration of ozonized sunflower oil to Wistar rats has produced changes in the urinary content of dicarboxylic organic acids (Jardines et al., 1998). An increment in urine of heptanedioic, octanedioic, octenedioic, nonanedioic, decenedioic and dodecanedioic acids has been observed. Heptanedioic acid (Pimelic acid) and nonanedioic acid (Azelaic acid) were the major increased dicarboxylic acids founded.More