Ozone Therapy Brain
The influence of ozone on the brain oxidation-reduction enzyme activity in normal condition and postresuscitation period
Irina V. Mukhina, Ludmila B. Snopova, Kate V. Dudina,
Roman D. Lapshin, Natalya N. Prodanetz
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia
The influence of the ozonated physiological saline (OPS) on the oxidative metabolism and lipid peroxidation system in brain tissue of the intact animals and in the reduction period after the clinical death has been studied. The OPS with the 135 µg/l ozone was infused to the intact rats at the background of the nembutal narcosis. The reperfusion period was studied at the 5 min clinical death model, the ozonised autoblood with the 80 µg/l ozone was intracarotid infused in the resuscitation period.
The influence of ozone both on the intact and hypoxic brain of rats in conditions of postresuscitation pathology was expressed in the neuron aerobic metabolism intensification and insignificant activation of peroxidation, causing the adaptive intensification of the cell antioxidant enzymatic system. In the period of reperfusion there is the pentaphosphate way activation, directed to provision of the synthetic processes in the brain at the postresuscitation period.
Now major attention is given to study of a bioenergy hypoxia as to a general purpose damaging part at many pathology conditions (1). The development of a histic hypoxia plays a major role in a pathogeny of reperfusion damages of organs. Excavation of a hypoxia promotes not only absence in the post reperfusion period of regeneration of a bloodflow and development of a phenomenon no-reflow at a microcirculation level, but also infringement of oxidation-reduction enzyme activity in cells of heart and, especially, brain.
As a result of the decrease of ability cells utilisation O2 in oxidation-reduction synthetic reactions of high-energy phosphates in tissues the glycolytic responses with accumulation of lactate prevail. Besides owing to infringement of work of a respiratory chain (2), O2 acting to cells at reperfusion, in the greater degree enters in response of free – radical oxidation (4). In this case application of drugs with antihypoxic properties not only would allow to sustain more effective aerobic energy metabolism in cells, but also to reduce a pathological activation of peroxide oxidation of lipids at appointment.
Variability in the pathogenesis of the postischemic disorders explain the lack of unified methods capable to prevent the development of reperfusion complications. Studying the pharmacological effect of the typical medical drugs we got interested in ozone as a substance