Ozone Therapy Methyl Linoleate
Effect of α-Tocopherol During in Vitro Ozonation of Methyl Linoleate. Its Implication in Ozone Therapy.
Maritza F. Díaz Gómez1; José A. Gavín Sazatornil2; Frank Hernández Rosales1 and Wilfredo Díaz1 Rubí.
1 Ozone Research Center. National Center for Scientific Research. Havana, Cuba. P.O. Box 6412, Havana, Cuba.
2University of the Laguna 38207, Tenerife, Spain.
Systemic ozone therapy is widely used as an oxidant therapy to restore many troubles and diseases. It is known that ozone therapy works through a transient oxidative stress that relays the beneficial effects to long distance from the application site. The convenience of supporting patients with antioxidants during systemic ozone therapy applications is now under discussion. We studied the reaction of linoleate (one of the main constituents of cellular membranes and plasma phospholipids) with ozone in presence or absence of α-tocopherol, in order to explore whether the combination of ozone and antioxidant has some effect on those ozonation products that produce the transient oxidative stress.
The reaction was followed by Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR) using 35 and 69 mg/L as ozone concentrations, and 50, 90, and 130 micromolar α-tocopherol concentrations with 0.0048 M of methyl linoleate. Ozonide (δ = 5.2 ppm) and aldehydes (δ = 9.63 ppm and δ = 9.74 ppm) intensities from 1H-NMR signals markedly decreased with α-tocopherol addition. When α-tocopherol is absent, the intensities from olefinic proton signals diminished with ozone concentration increment; however, with α-tocopherol in the mixture a smaller decrement was achieved. No detectable signals were found with the ozonation of α-tocopherol without methyl linoleate in the reaction mixture. These results suggest that α-tocopherol reacts with ozonation products from the reaction of ozone with methyl linoleate. This fact point out that antioxidant supplementation during systemic ozone therapy (major and minor autohemotherapy, rectal insufflation, etc) can be detrimental in achieving the needed transient oxidative stress.
Ozone Therapy Female Genital Organs
The New Method of Treatment of Inflammatory Diseases of Lower Female Genital Organs
Guennadi O. Gretchkanev1, Tatyana S. Katchalina2, Olga V. Katchalina3, El-Hassoun Husein4
1,2,3Department of Obstetrics and Gynecology, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
4 Russian Association of Ozone Therapy, Jordan
For treatment of 40 patients with non-specific colpitis we used a method of streaming ozone therapy with assistance of the ozone generator MEDOZONS-BM and special attachment on the basis of vaginal speculum. The irrigation with an ozone-oxygen mixture with ozone concentration of 2000 mcg/L was carried out within 5 minutes after preliminary moistening of mucosa with sterile distilled water.
In the course of investigations it was established that along with a positive clinical effect ozone therapy caused an improvement in vaginal local immunity indices – a credible increase in lysozyme in 70% of patients, a decrease in IgG level in 60% of patients, stabilization of cervical mucus myeloperoxidase in 80% of patients.
The problem of infectious diseases and dysbiosis nowadays acquires particularly great importance that is connected with growing ecological problems of urbanized society, exposure to various adverse factors having influence on the immunity status in general as well as micro biocenosis of human body. It is established that the pathological processes of vaginal micro biocenosis are caused by such stress factors as treatment with antibiotics (at local and systemic level), hormones, cytostatic preparations, X-ray therapy, particularly on the background of endocrinopathies (most of all, diabetes), anemia, in case of birth defects of genital organs, the use of contraceptives and so on (1). It is convincingly demonstrated that the disturbances of a quantitative correlation between the association of vaginal micro biocenosis on the background of local immunity changes lead to clinical appearances of vaginal infection process, the most frequent of them are non-specific vaginitis and bacterial vaginosis (1,3). Bacterial vaginosis is detected in 24% of practically healthy women and in 61% of patients with recidivating colpitis of nonspecific etiology (2), frequency of nonspecific vaginitis, in reference to different authors, reaches 40 to 50%. The extensive use of new antibiotics does not solve the problem, but in some cases aggravates it by inducing the disturbances of the local and general immunity, by suppressing saprophytic flora, by causing the formation of antibiotic-resistant forms of microorganisms, by creating favourable conditions for infection that increases frequency of recidivating colpitis. In this connection, our attention is drawn to the methods of treatment which can be characterized as highly effective against the most frequent causative agents of infection, able to reduce antibiotic and any other drug therapies and able to restore the normal vaginal biocenosis.
Medical Ozone Endometritis
The Treatment of Endometritis in Combination with Ozone therapy
Guennadi O. Gretchkanev1, Tatyana S. Katchalina2, Olga V. Katchalina3, N.N. Saifieva4,
1,2,3,4 Department of Obstetrics and Gynecology, Medical Academy of Nizhny Novgorod,
Pl. Minina 10/1, 603005 Nizhny Novgorod, Russia
5 Russian Association of Ozone Therapy, Jordan
The inflammatory diseases of female genital organs are numbered among the most frequent pathologies of fertile women. In order to increase the efficiency of treatment and to avoid possible side effects we have used intrauterine irrigations with ozonated distilled water in case of acute endometritis. This method of treatment has been provided to 15 patients with postnatal and post-abortive endometritis. Based on the received results, we have drawn a conclusion that in the course of treatment all patients showed an improvement in general state, body temperature returned to normal, symptoms of intoxication decreased, sleep and appetite got better, pain syndrome disappeared faster, pathological discharges were eliminated. The results of laboratory investigations have demonstrated a credible decrease in ESR, normalization of leukocyte number, a decrease in circulating immune complexes.
Thus, for treatment of endometritis we may recommend the given method of ozone therapy to be used in combination with a basic anti-inflammatory therapy.
Endometritis is being still numbered among the most frequent pathologies of fertile women. But in spite of considerable success achieved in the treatment of inflammatory, incl. gynecological diseases, the problem of efficient treatment of endometritis is not solved yet. It can be explained as follows:
- high frequency of complications due to endometrial manipulations (therapeutic abortion, diagnostic endometrectomy and so on) that mostly appear then in the form of acute endometritis;
- considerable risk that the infectious-inflammatory process localized in uterus will generalize;
- numerous side effects of medicines to be used within the traditional treatment of endometritis.
In practical gynecology along with a basic therapy (antibacterial, desensitization, vitamins, sedative remedies) antiseptic preparations have been widely used in the form of intrauterine applications. The aim of this manipulation is to improve the removal of pyo-necrotic masses as well as to produce a medicinal effect directly in the focus of inflammation that however
can cause a number of side effects.
Ozone Therapy Asthma
Changes in Glutathione Antioxidant Pathway Components, HLA-DR and IgE in Blood from Asthma Patients Treated with Ozone Therapy.
Frank Hernández, Idania Alvarez, Ivonne Corcho and Maritza González
Department of Biomedicine, Ozone Research Center, National Center for Scientific
Research, A.Postal 6412, Ciudad de La Habana, Cuba.
We have studied the behavior of the erythrocyte glutathione antioxidant pathway, the HLA-DR molecule expression and the serum IgE level in patients suffering reactive asthma, whose were treated with ozone major autohemotherapy or rectal insufflation. A subgroup of patients having high levels of serum IgE (> 250 IU/mL) presented high level of HLA-DR expression (> 35 %) and lower concentrations of erythrocyte GSH (<2.00 µmol/g.Hb) and GPx (< 11.62 IU/g.Hb) as a basal state before the beginning of ozone therapy. GST and GR were in the normal range. After to complete 15 sessions of ozone autohemotherapy applications (dose of 8 mg each session), all parameters in the glutathione antioxidant pathway were highly elevated and, IgE and HLA-DR levels decreased markedly respect to the initial values.
With ozone rectal insufflation (20 sessions at an ozone dose of 10 mg each one), similar results were achieved although the changes were not so significant as in authohemotherapy. When the analysis was done considering all patients (with or without initial high level of IgE) the changes in GSH, GPx and GST were sligthly higher after autohemotherapy without changes in GR and IgE, but HLA-DR expression significantly decreased. These findings support a role for IgE producing oxidative stress and inflammation in allergenic asthma. On the other hand, ozone therapy proved to be effective in this disease due, likely, to its regulation on glutathione antioxidant pathway which lead to a control in the oxidative stress present in the asthma patients and a change in the cytokine pattern.